Saturday, 3 March 2012

5.12 Restriction and Ligase Enzymes

Describe the use of restriction enzymes to cut DNA at specific sites and ligase enzymes to join pieces of DNA together.

  1. The restriction enzyme is able to cut the DNA, the restriction enzyme cuts the DNA at a particular location, and this location is identified by the base sequence
  2. The ligase enzyme is able to join the two pieces of DNA together.

These are commonly used with genetical engineers and bio engineers.


  1. Hi there! glad to drop by your page and found these very interesting and informative stuff. Thanks for sharing, keep it up!
    Note that, originally, biochemical nomenclature distinguished synthetases and synthases. Under the original definition, synthases do not use energy from nucleoside triphosphates (such as ATP, GTP, CTP, TTP, and UTP), whereas synthetases do use nucleoside triphosphates. It is also said that a synthase is a lyase (a lyase is an enzyme that catalyzes the breaking of various chemical bonds by means other than hydrolysis and oxidation, often forming a new double bond or a new ring structure) and does not require any energy, whereas a synthetase is a ligase (a ligase is an enzyme that binds two chemicals or compounds) and thus requires energy.
    Enzymatics' high-concentration T4 DNA ligase in combination with the 2X Rapid Ligation buffer greatly stimulates the rate and efficiency blunt-end ligation, therefore long incubations (>10 minutes) are NOT recommended and can greatly reduce the transformation efficiency of ligation products. In order to maximize transformation efficiency of the correct insert/vector combination, the following protocol is recommended.

  2. sorry pal but you didnt bring anything new you just copied it !!!!!!!!
    i want their uses